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KMID : 1098420040120060494
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Korean Journal of Medicinal Crop Science
2004 Volume.12 No. 6 p.494 ~ p.499
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Long-Term Cold Storage and Plant Regeneration of Suspension Cultured Somatic Embryos of Eleutherococcus senticosus Maxim
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Li Cheng-Hao
Lim Jung-Dae
Heo Kweon
Kim Myong-Jo
Lee Chan-Ok
Lee Jae-Geun
Cui Xue Shu
Yu Chang-Yeon
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Abstract
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A method for long-term conservation of somatic embryos of Eleutherococcus senticosos was described. Suspension cultured globular somatic embryos were successfully conservated for 36 months at 4^{circ}C. The embryos resumed growth within two weeks when returned to MS liquid medium containing 0.2;mg/{ell}. 2,4-dichlorophenoxy acetic acid. The optimal condition for cell proliferation was achieved when somatic embryos cultured at 32^{circ}C in 1/3 MS liquid medium, and about 1.2 g of embryogenic cell was induced from 150 globular embryos after 6 weeks of suspension culture. The embryogenic cells produced from these somatic embryos exhibited normal plant regeneration on auxin-free medium.
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KEYWORD
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Eleutherococcus senticosus, suspension culture, somatic embryo, 2, 4-dichlorophenoxy acetic acid
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